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KMID : 0364820080440020140
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Korean Journal of Microbiology
2008 Volume.44 No. 2 p.140 ~ p.146
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Secretion of Cytokine Stimulating Intercellular Adhesion Molecule-1 Expression from THP-1 Cells Infected with Human Cytomegalovirus
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Kim Mi-Suk
Lee Chan-Hee
Yi Hyun-Ah
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Abstract
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Human cytomegalovirus (HCMV) stimulates the expression of intercellular adhesion molecule (ICAM-1) on
the surface of monocytic THP-1 cells. Stimulation of ICAM-1 did not require HCMV gene expression since
UV-inactivated HCMV (UV-HCMV) was able to induce ICAM-1 expression. ICAM-1 expression was also
stimulated in uninfected THP-1 cells which were fed with culture supernatant of HCMV-infected THP-1 cells.
Co-culture experiment using trans-well insert supported that HCMV-infected THP-1 cells secreted some cytokine(
s) stimulating ICAM-1 expression. The stimulation of ICAM-1 by HCMV-infected cell culture supernatant
appears to involve NF-κB pathway. Culture supernatant from THP-1 cells infected with UV-HCMV, whose
gene expression was abrogated, failed to stimulate ICAM-1 expression on naive THP-1 cells. Thus, HCMV
gene expression seems to be required in secretion of cytokine(s) stimulating ICAM-1 expression.
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KEYWORD
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HCMV, ICAM-1, supernatant
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